The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
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Info-enriched characterization of cell populations. Morphological details from images can increase to your richness of flow cytometry facts. One example is, the figure demonstrates an usually common apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Each individual population to reveal morphologically distinct features. These insights couldn't are already acquired from multiplex staining by yourself.
No matter whether you’re a newbie or a professional, explore our methods to advance your analysis and skills in flow cytometry.
Incorporate acoustic focusing technological know-how with high-parameter Assessment for adaptable spectral unmixing and standard workflows.
Learn new insights into your experimental details by visualizing populations utilizing merged fluorescence and morphology info. Our application interface wizards empower downsampling, concatenation (merged documents) and dimensionality resources that happen to be built to the application for an intuitive, streamlined knowledge Assessment encounter.
"An image is not rather worth 1000 dots, but it really actually really helps to see photographs. It is great to determine illustrations or photos that correlate with staining. For example, AnnexinV-constructive cells which might be morphologically distinctive than healthier cells, or CD14+ monocytes which are greater and even more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it can be illuminating to determine the amount of doublets slip through standard singlet gates, and great to validate the standing of surprising double-optimistic functions in my Investigation."
Optimizing gating strategy. Even robust guide singlet gating is error-inclined and remains a subjective determination position in Practically all flow cytometry assays. Imaging can be used to verify and modify gates to incorporate only solitary cells of fascination.
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The Attune NxT cytometer may be the trusted workhorse instrument your lab can count on for dependable and trusted performance. The Attune CytPix model includes a substantial-pace brightfield digicam and AI-driven graphic analysis program, with modern upgrades like device Understanding instruments and an intuitive user interface, enabling enhanced data precision, precision, and a lot quicker insights into sample biology.
Probably most significantly, these situations include cells of Plainly unique phenotypes which could lead to incorrect conclusions with regards to double good gatherings (especially in unusual populations).
AI-pushed automated image Investigation With all the Attune Cytometric Software package interprets function options into distinct morphology parameters that could be coupled with common fluorescence and scatter parameters to reveal extremely reproducible population improvements and unique subsets.
We previously demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Let’s evaluate just the populace of best interest, the double optimistic gatherings, to learn more. We will now use extended morphological parameters (right here, circularity and skewness of depth) to additional examine the capabilities of such populations and refine gating on these activities, expanding data robustness.
Leverage the strength of advanced technological innovation and innovation to speed up your discoveries. Have faith in in the Attune Flow Cytometer family to provide the trustworthiness, speed, and accuracy that established your work apart.
No clean/no lyse leukocyte Investigation with imaging. Cells have been acquired from 24-hour-previous blood by dilution in 1 mM EDTA ( Skilled UX/UI permits users to prepare AI versions for expanded purposes. (A) The “Prepare Design” aspect gives annotation applications and real-time design teaching accuracy.
Gating on these extended parameters allows you to swiftly and precisely detect populations of more info fascination and ensure gating strategy with little if any manual evaluate. The Attune computer software provides entry to condition-of-the-artwork equipment Finding out analysis resources in just an expertly created consumer interface, rendering it exceptionally simple to use.
The Attune Xenith cytometer is really a significant-parameter spectral analyzer, furnishing each spectral unmixing and conventional flow abilities for thorough and multipurpose cell Evaluation.
The Attune CytPix Flow Cytometer makes it possible for customers to visualize samples in the course of acquisition, validate the precision of gating methods, and help success with responsible documentation for publication reasons.
Right here, we exhibit that by gating strategies utilizing impression-based mostly quantitative parameters can distinguish interacting cells from coincident events to more precisely review interacting cells.